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Chromatography Columns & Cartridges

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Column chromatography refers to the science of separating organic compounds into distinct components, either to analyze their content or to isolate individual compounds for purification purposes. The process consists of stationary and mobile (or eluent) phases. A glass tube, or column, containing silica gel or another inert compound as an absorbent comprises the stationary phase. Scientists then fill the silica column with a solvent or mixture of solvents, or eluents, in the mobile stage. The two interact to create a partitioning flow that separates the eluents into analytes, or the constituent components, for analysis or purification. Staples® carries these items and other scientific supplies.

Column Chromatography Now Consists of Advanced Instrumentation
Chromatography equipment containing hi-tech column configurations has replaced manual glass-tube chromatography in laboratories that have to undertake mass purification and separation procedures. One of the machines is a high-performance liquid chromatography (HPLC), which pumps a sample mixture from a solvent at high pressure through a silica-embedded column, also known as chromatographic packing material. The HPLC process can separate and identify compounds in any sample that is liquid-dissolvable. The packing material consists of chromatography cartridges and HPLC columns that are able to isolate trace concentrations down to parts per trillion, ideal for pharmaceutical, environmental, forensic, and chemical applications.

Column Chromatography Also Consists of Hydrophilic Interaction Liquid Chromatography (HILIC)
A related chromatographic application consists of a process called ligand exchange, where a ligand, or molecular substance, bonds with another molecule to form a substitute compound. This HILIC process also uses chromatography cartridges for the separation process, many of which are useful for isolating disaccharides or tri-saccharides in the laboratory. This process enables scientists to analyze the formation of carbohydrates.

What Is the Relationship Between Column Chromatography and Mass Spectrometry?
Many silica columns serve a dual purpose between liquid chromatography (LC), where the columns help isolate the components of a sample, and mass spectrometry (MS), in which a mass spectrometer turns the sample molecules into charged ions. The MS process is able to detect and identify with greater precision than with LC-sample analysis alone. The LC/MS process uses chromatography cartridges for a variety of clinical applications, including biochemical genetics, providing extremely accurate information about the molecular weight, structure, and quantity of specific sample components. Many cartridges also have cross-application in the LC process, working with the evaporative light scattering detector (ELSD) that HPLCs use to identify compound components digitally for analysis.

How Large Are Chromatography Cartridges?
The cartridges for HPLC, HILIC, LC-MS, and ELSD applications come in different lengths, suitable for specific applications. They range from 30 to 150 millimeters in length. Each also has different pore sizes, for purification of different particles depending on their molecular weight. Pore sizes can range from 6 to 9 nanometers.

How Are Chromatography Cartridges Packaged?
The columns are delicate, so they’re packaged individually in Styrofoam containers conforming to their shape, with tight-fitting lids. After use in column chromatography applications, they should be washed and dried thoroughly, and returned to the packaging for protection and subsequent reuse.
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Restek Corporation Allure C18 HPLC Column
Item: 1602601 / Model : 9164552-700 EA
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  • Length: 30 mm
  • Used for LC/MS and ELSD
  • Pore Size: 60
554.99 $554.99
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